Journal: bioRxiv

Article Title: Cell-derived ECM loaded electrospun Polycaprolactone/Chitosan nanofibrous scaffolds for periodontal regeneration

doi: 10.1101/2023.03.30.534964

Figure Lengend Snippet: Characterization of decellularized extracellular matrix (dECM) derived from human periodontal ligament stem cells (PDLSCs). A) Immunofluorescence staining images of fibronectin (FIB, green), collagen I (COL I, red), laminin (LAM, red), asporin (ASP, red), osteopontin (OPN, red) and osteocalcin (OC, red) before and after decellularization DAPI staining (blue) revealed the absence of nuclei after decellularization. Scale bar 100 μm. B) DNA content present in PDLSCs and dECM. C) Quantification of sulphated glycosaminoglycans (sGAGs) in PDLSCs and dECM. D) Content of collagen present in PDLSCs and dECM. Three different samples were used in each quantification assay (N=3) for both conditions; *** p < 0.001.

Article Snippet: The primary antibodies, including rabbit polyclonal collagen I (1:200, Abcam), rabbit polyclonal asporin (1:100, Thermo Fisher Scientific), mouse monoclonal osteopontin (1:100, Thermo Fisher Scientific), mouse monoclonal osteocalcin (1:50, R&D Systems), rabbit polyclonal periostin (1:100, Abcam) and rabbit polyclonal cementum protein 1 (1:100, Thermo Fisher Scientific) prepared in a solution of 0.3% Triton X-100, 1% BSA, 10% FBS (in PBS) were added into the samples and incubated overnight at 4°C.

Techniques: Derivative Assay, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: Cell-derived ECM loaded electrospun Polycaprolactone/Chitosan nanofibrous scaffolds for periodontal regeneration

doi: 10.1101/2023.03.30.534964

Figure Lengend Snippet: Immunofluorescent staining images of collagen I (COL I, red), asporin (ASP, red), osteopontin (OPN, red), osteocalcin (OC, red), periostin (POSTN, red) and cementum protein 1 (CMP, red) expressed by PDLSCs cultured on PCL, PCL-CTS and PCL-CTS-ECM electrospun scaffolds for 21 days under osteogenic differentiation conditions. Nuclei were counterstained with DAPI (blue). Scale bar 100 μm.

Article Snippet: The primary antibodies, including rabbit polyclonal collagen I (1:200, Abcam), rabbit polyclonal asporin (1:100, Thermo Fisher Scientific), mouse monoclonal osteopontin (1:100, Thermo Fisher Scientific), mouse monoclonal osteocalcin (1:50, R&D Systems), rabbit polyclonal periostin (1:100, Abcam) and rabbit polyclonal cementum protein 1 (1:100, Thermo Fisher Scientific) prepared in a solution of 0.3% Triton X-100, 1% BSA, 10% FBS (in PBS) were added into the samples and incubated overnight at 4°C.

Techniques: Staining, Cell Culture

Journal: Virology

Article Title: Zika virus infection of adult and fetal STAT2 knock-out hamsters.

doi: 10.1016/j.virol.2017.04.013

Figure Lengend Snippet: Fig. 2. ZIKV immunohistochemistry of testes of STAT2 KO hamsters s.c. injected with ZIKV. ZIKV immunofluorescence (mouse monoclonal antibody to ZIKV NS1 protein) of testes from ZIKV-infected hamsters #01 (A-C,E-G), #61 (I-K), and sham (M-O) at 10 dpi, and ZIKV-specific peroxidase (mouse anti-flavivirus group antigen) catalyzed staining of Vero cells co-cultivated with homogenized testes from ZIKV-infected hamster #01 (Q) and sham (R). ZIKV-specific immunoperoxidase staining counterstained with hematoxylin of testes tissue (D,H,L,P). Solid arrow head – spermatogonia morphology; Solid arrow – Sertoli cell morphology. Scale bar =50 µM.

Article Snippet: Either mouse anti-flavivirus group antigen monoclonal antibody (Millipore, CA) or mouse monoclonal antibody to ZIKV NS1 protein (Aalto Bio Reagents, Dublin, Ireland) were incubated on the tissues for overnight.

Techniques: Immunohistochemistry, Injection, Infection, Staining, Immunoperoxidase Staining

Journal: Virology

Article Title: Zika virus infection of adult and fetal STAT2 knock-out hamsters.

doi: 10.1016/j.virol.2017.04.013

Figure Lengend Snippet: Fig. 4. ZIKV and histopathology of placental tissues from STAT2 KO hamsters s.c. injected with 500 CCID50 of ZIKV 8 dpc. Tissues were collected at 14 dpc (6 dpi). ZIKV immunofluorescence (mouse monoclonal antibody to ZIKV NS1 protein) using peroxidase-catalyzed staining counter-stained with hematoxylin (A-E). Scale bar =50 µM.

Article Snippet: Either mouse anti-flavivirus group antigen monoclonal antibody (Millipore, CA) or mouse monoclonal antibody to ZIKV NS1 protein (Aalto Bio Reagents, Dublin, Ireland) were incubated on the tissues for overnight.

Techniques: Histopathology, Injection, Staining

Journal: Neural Regeneration Research

Article Title: The mechanism of Naringin-enhanced remyelination after spinal cord injury

doi: 10.4103/1673-5374.202923

Figure Lengend Snippet: Effect of naringin on GSK-3β phosphorylation and β-catenin levels in rats 4 weeks post-injury. (A) Western blot assay of GSK-3β phosphorylation and β-catenin levels in different groups. β-Actin was used as a loading control. (B, C) The level of GSK-3β phosphorylation (B) and β-catenin protein (C). Three separate experiments produced similar results. * P < 0.05, vs . vehicle group (SCI + vehicle). Data are expressed as the mean ± SD ( n = 7; one-way analysis of variance and Tukey's post hoc test). GSK-3β: Glycogen synthase kinase-3β; p-GSK-3β: phosphorylated glycogen synthase kinase-3β.

Article Snippet: The antibodies used were: polyclonal rabbit anti-NG2 antiserum (1:1,000; Millipore, Bedford, MA, USA), monoclonal mouse anti-Olig2 (1:1,000; Abcam, Cambridge, MA, USA), monoclonal mouse anti-NKx2.2 (1:1,000; Abcam), monoclonal mouse anti-CNPase (1:500; Sigma-Aldrich), polyclonal goat anti-Ser9-pGSK-3β (1:200; Sigma-Aldrich), monoclonal mouse anti-β-catenin (1:500; Abcam) and β-actin (1:10,000; Sigma-Aldrich).

Techniques: Western Blot, Produced